Biologia Plantarum 1998;40(4):515-522.
In vitro multiplication of Beta vulgaris L. throughout excised shoot tips.
Rady MR.
The excised shoot tips of Beta vulgaris L. cvs. Ras poly, Gala and Lola were inoculated aseptically on Murashige and Skoog's (MS) medium with 0.25 mg dm(-3) alpha-naphthaleneacetic acid (NAA) and 1.00 mg dm(-3) 6-benzylaminopurine (BAP). They produced multiple shoots at relatively high frequency. For shoot multiplication, a concentration of 1.00 mg dm(-3) NAA and 0.25 mg dm(-3) BA in the culture medium was found to be optimum for all the cultivars. MS medium supplemented with 3.0 mg dm(-3) 3-indolebutyric acid (IBA) and 0.02 mg dm(-3) N-isopentenylaminopurine (2ip) was found most effective for rooting of micropropagated shoots. In vitro produced plantlets were successfully transferred to the soil. The percentage of survival was more than 95 % and micropropagated plants showed no morphological differences from those grown naturally.
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